Diffusion and mobility. For a dilute isolated sphere in a Newtonian fluid, Stokes's law gives the drag magnitude , where is the dynamic viscosity and is the hydrodynamic radius. The mechanical mobility is therefore ; the translational hydrodynamic mobility matrix is .
To relate mobility to diffusion, put the particles in a weak external potential energy . Their drift speed is . For number density , the sum of drift and Fick's first law fluxes isAt thermal equilibrium, the Boltzmann distribution gives , and the net flux is zero. Substituting into yields . Thus the Stokes–Einstein relation isHere is the Boltzmann constant and is absolute temperature. The assumptions include a no-slip approximately spherical particle, a continuum fluid, a small particle Reynolds number, and negligible hydrodynamic interactions with other particles or boundaries.
Gravitational sedimentation. The buoyant force must be included, since the mass of displaced water is comparable to the protein mass. Using givesso the buoyant mass in the stated spherical model isThe drag coefficient is . At terminal speed, , soIgnoring buoyancy would give and would not use the supplied water density. This calculation treats the stated radius as both the hydrodynamic radius and the radius determining displaced volume; a real hydrated protein can require a separately measured partial specific volume.
Electrical drift. In the specified simple electrophoresis model, terminal force balance is . Thereforewhere is the electrophoretic mobility and the elementary electric charge. For the given positive beta-lactoglobulin charge,in the direction of the electric field. This exceeds the gravitational sedimentation speed by about .
Separation time. The two populations have equal drag coefficients because their radii are equal. Their difference in drift speed is therefore set by one elementary charge:Starting with coincident band centres, their separation is . HenceAt that time the original band has travelled and the mutant . Their masses do not enter this ideal electrical force balance: equal-radius particles with different masses but the same charge would have equal electrophoretic mobility in this model.
Practical discrimination. A one-charge difference is enough for the stipulated separation, so the method is sensitive to mutations that change effective charge or friction. It cannot distinguish every mutation: substitutions that preserve charge and hydrodynamic size need not separate. Buffer pH changes protein ionization, and realistic electrophoresis also involves the ionic atmosphere, electro-osmosis, walls or gel interactions, and finite band widths.
For example, the Stokes–Einstein relation at gives . For an initially narrow band, diffusive broadening of electrophoretic bands givesat the calculated separation time. Thus the centres are about diffusion standard deviations apart, rather than forming infinitely sharp bands. The stated centre-separation criterion is achievable in the model, but actual resolution also depends on the loading width and other dispersion mechanisms.
Equivalent centrifugal acceleration. For the original protein to attain its electrical drift speed by sedimentation, its buoyant centrifugal force must equal :ConsequentlyThis is the acceleration at the protein's position in the rotating solution; no rotation rate can be assigned without a centrifuge radius. If the intended comparison is instead the relative separation speed of the two bands, use , since their displaced volumes are equal. Matching then gives . In that separation experiment the heavier original protein moves faster centrifugally, whereas the more highly charged mutant moves faster electrically.
Choose a coordinate measured from the sphere centre towards increasing distance from the block, so an atom in the sphere is at height . Summing the atom–block potential energy with the sphere's number density givesFor , a uniformly valid Taylor series over the sphere isThe odd moments vanish by reflection symmetry. The sphere volume is , and its second moment isConsequentlyThe first finite-radius correction is therefore . There is no relative correction because the first moment about the centre is zero. The leading term is just the number of sphere atoms times the potential at its centre; the correction increases the attraction because the nearer atoms contribute more strongly than the farther atoms lose.
This is the far-field sphere-plane dispersion energy, with Hamaker constant . It must be distinguished from the near-contact sphere-plane dispersion force, whose small parameter is the surface gap divided by the radius. Here is explicitly the centre-to-plane distance.
Without stabilization, the potential energy is predominantly attractive outside the molecular core and drops into a deep primary minimum. With the charging treatment, the DLVO theory sum develops a positive barrier between distant particles and that primary minimum. The barrier must be large compared with to inhibit aggregation on the experimental timescale. A shallow secondary minimum can remain at larger separation, depending on parameters.
Attractive and electrostatically stabilized colloidal pair potentials
. Original illustrative curves for two equal spheres. The unstabilized curve uses the nonretarded sphere–sphere attraction; the stabilized curve adds screened electrostatic repulsion. The molecular core is excluded and the deep near-contact well is clipped by the plotting range. Potential depths and screening parameters are illustrative, not data supplied by the paper. Details.The horizontal axis is the surface gap, so the molecular exclusion boundary is at its origin. Both interactions tend to zero at large separation. Raising salt concentration shortens the Debye–Hückel screening length and can reduce the barrier, allowing particles to stick again.
In a magnesium chloride chemical solution, the principal pair interactions are hard sphere exclusion, repulsion of overlapping electric double layers, and attractive Van der Waals forces. The positive surface attracts chloride counterions and repels magnesium coions; mobile ions screen the bare Coulomb's law interaction. The competition between screened electrostatic repulsion and Van der Waals force attraction is described by DLVO theory.
For well-separated identical weakly charged spheres, the approximate repulsive pair potential energy, at centre separation , isIt is a screened Coulomb pair potential. An attractive Hamaker constant adds a negative short-range contribution, while overlap is forbidden. Very near contact, solvation and molecular surface structure can also matter. No polymer-induced interaction should be assumed before polymer is added in part (f).
Past exam of the symmetry and relativity course of the University of Oxford 2019 1 e Solution 2026-10-25
For causal reachability on a spatial circle, put and use a representative of the spatial circle. The lower-left panel of part c is a fundamental strip with its vertical edges identified. On the covering space the charged world line is still the solution of part b; on the quotient it isA jump between opposite strip edges is a coordinate seam, not a discontinuity of the physical world line. At its th complete lap, , soFor , the speed increases on each return while remaining below . Its kinetic energy increases byThere is no single-valued electrostatic potential energy on a circle: the nonzero electromotive force is . A globally defined magnetic vector potential one-form instead makes the driving explicit. In an embedded circuit, Faraday's law realizes this electromotive force through changing magnetic flux. More generally an external apparatus maintaining the field supplies the increasing particle energy. In an isolated dynamical field-plus-particle system, backreaction would change the field. Thus this is not a cyclic machine extracting work from a conservative potential, and it does not violate conservation of energy.
Past exam of the symmetry and relativity course of the University of Oxford 2019 4 b Solution 2026-10-25
Restoring units, the decaying Yukawa potential is with range . For ,The geometric factor belongs in the potential ratio; the exponential alone is the additional suppression relative to massless exchange. Using givesThe following are ratios of potential magnitudes to the magnitude at , with the same coupling held fixed:
| Mediator | ||
|---|---|---|
| Massless scalar |
For example, the pion's nuclear exponential factor is , whereas the Higgs nuclear exponent is about . The huge atomic suppressions are most reliably reported through logarithms, not through floating-point exponentiation that silently underflows to zero. These are potential energy ratios, not force ratios or absolute comparisons of different interaction strengths.
Take positive toward positive from the downward vertical, as in the source diagram. The mass has position and velocityThus and . Expanding the squared speed givesThe prescribed support coordinates are functions of time, rather than additional dynamical variables. Terms depending only on time do not affect the Euler-Lagrange equation for , but have been retained here to obtain the inertial-frame kinetic and potential energies directly.
Write the bead position in Cartesian coordinates as . Differentiation gives , while the free particle has . Thus the kinetic energy is the sum of these squared speeds weighted by half the respective mass. The squared spring length isA spring obeying Hooke's law with zero natural length has potential energy . Consequently , and deleting the additive constant givesThe spring length is the straight separation of its endpoints, not distance measured along the helix. The fixed frictionless constraint is already incorporated by using the generalized coordinate ; it supplies no extra potential energy.
Past exam of the theoretical physics I course of the University of Cambridge 2022 1 b Solution 2026-10-18
Let and be the lower and upper azimuthal angles, measured from a common fixed direction. The squared spring length isBy Hooke's law, a spring with zero natural length has potential energy . ThereforeConstant gravitational energies can be omitted. It is useful to define the moments of inertia , and the angular coupling . The part of the potential that affects motion is simply . In particular, changes the constant spring energy but not the angular equations, because the spring has zero natural length.
Past exam of the theoretical physics I course of the University of Cambridge 2022 1 e Solution 2026-10-18
Let be or , and write the angular displacements as . To quadratic order the restoring potential energy is . The linearized normal mode equation isThe determinant gives . Thus the normal modes of two spring-coupled coaxial rotors areFor the aligned family, the relative mode oscillates with positive frequencyFor the antipodal family, , so that displacement has solutions and the equilibrium is unstable.
The common zero mode has . It describes a neutral displacement or uniform common rotation, not a restoring oscillation. Thus the aligned family is stable with respect to relative motion and has orbital stability modulo common rotations. A particular static point is not strictly Lyapunov stable against unrestricted small velocity perturbations: arbitrarily small nonzero eventually moves the common angle far from that point. With , the common angle is fixed and the aligned relative equilibrium point is stable. This distinguishes the symmetry-induced drift from the exponential instability of the antipodal family.
Past exam of the theoretical physics II course of the University of Cambridge 2017 2 a Solution 2026-10-18
Complete the square in the potential energy. With ,The quantum harmonic oscillator is translated without changing its level spacing. If is its normalized unforced energy eigenstate, thenEquivalently, . The ground state position is centered at , and its position uncertainty remains .


