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The Airy disk is the central bright region, up to the first dark ring, in the scalar diffraction pattern of a uniformly illuminated, unobscured circular aperture. The whole pattern includes the weaker surrounding rings; its radius is distinct from its full width at half maximum.

For a circular optical pupil of diameter $D$ under normal, uniform monochromatic plane wave illumination, Fraunhofer diffraction givesHere $I_0$ is the on-axis light intensity, $\theta$ is the angle from the optical axis, $\lambda$ is the wavelength in the propagation medium, and $J_1$ is the order-one Bessel function of the first kind. The paraxial approximation replaces $\sin \theta$ by $\theta$, measured in radians. The first positive zero $j_{1,1}=3.831705970\dots$ givesThis is the angular radius of the Airy disk; the angular diameter is twice as large. The Airy disk derivation explains the origin of the Bessel function of the first kind and of the numerical coefficient.^[ref]

The image and both radial curves show the same calculated light intensity, divided by its peak. The image's logarithmic scale makes the weak rings visible; its colour is a display choice. The linear curve shows how small the ring peaks are compared with the centre. A dark ring marks a zero of wave amplitude, produced by cancellation across the optical pupil; it is not the edge of all transmitted light. The first Airy disk contains about $83.78\%$ of the total radiant flux, as calculated in Airy disk normalization and encircled energy.

For an original design example, take an ideal optical lens in air with focal length $f=100\mathrm{mm}$, circular optical pupil diameter $D=5.00\mathrm{mm}$ and wavelength $\lambda =550\mathrm{nm}$. In the focal plane, the radial coordinate $r\simeq f\theta$ givesThe first-zero diameter is $26.8327\mu \mathrm{m}$. Solving $P(u_{1/2})=1/2$ gives $u_{1/2}=1.616339948\dots$, so the diameter measured by full width at half maximum is onlyThus a quoted “spot diameter” is incomplete without its definition. Doubling $D$ halves both widths at fixed wavelength and focal length; increasing magnification alone changes the image scale without improving object-space discrimination.

The Airy disk supplies a calculable reference for a point spread function, allowing diffraction to be distinguished from defocus and optical aberrations. Its beauty is that one circular boundary determines an entire sequence of bright and dark rings through a Fourier transform. The ideal formula assumes an unobscured pupil, uniform illumination, ideal optical focus, a single wavelength, and a scalar description with small output angles. A blocked centre, nonuniform pupil transmission or a significant pupil wave phase error changes the response. High numerical aperture may require polarization of an electromagnetic wave and a vector field description. The circular-aperture diffraction laboratory experiment tests the far-field pattern directly; the Rayleigh criterion uses its first zero to define a two-source convention.

Opening the pinhole increases accepted in-focus and out-of-focus photons; closing it strengthens confocal optical sectioning while discarding some useful in-focus photons too. In the synthetic model, $\alpha =0.5,1.5,4$ transmits approximately $0.1175,0.6753,0.9997$ of the collected in-focus emission. At $t=2$ the corresponding peak-normalized signals are about $0.0420,0.0597,0.1597$. These are calculated fractions under stated assumptions, not instrument measurements.

An Airy unit conventionally specifies a pinhole diameter equal to the first-zero diameter of the emission Airy disk in that plane. For an ideal circular pupil,where $M$ is the total specimen-to-pinhole magnification, ${\lambda }_{\mathrm{em}}$ the emission wavelength in vacuum, and $\mathrm{NA}$ the objective numerical aperture. A software setting needs its specified wavelength and relay magnification; it is not a universal micrometre diameter. ^[ref]

For ideal independent counting, accepted source and background means $S$ and $B$ give photon shot noise with variance $S+B$ and signal-to-noise ratio in photon counting $S/\sqrt{S+B}$. Closing a pinhole need not improve that ratio: it depends on how both $S$ and $B$ change. Increasing exposure or excitation to replace discarded photons can cause bleaching, an irreversible loss of fluorescent emission capacity, or damage. Detection rejection occurs after excitation and therefore does not undo excitation outside the selected depth. Such material can absorb illumination and emit photons even when their emission is omitted from the recorded image.

Diffraction-limited resolution is a scale for distinguishing details with a specified ideal diffraction response and a specified criterion. For two equally bright, mutually incoherent optical point sources imaged through a uniformly transmitting, unobscured circular optical pupil, the Rayleigh criterion places the centre of one Airy disk at the first zero of the other. At small angles in air this separation isHere $D$ is pupil diameter, $\lambda$ is wavelength in air, and $j_{1,1}$ is the first positive zero of the order-one Bessel function of the first kind. This is the radius to the first zero, not the diameter of the central spot. Fraunhofer diffraction gives the radial peak-normalized light intensityThe removable value at zero follows from $J_1(q)\sim q/2$. The pupil Fourier transform reduces to a radial integral by circular symmetry, which produces the Bessel function of the first kind; its first zero supplies the factor $1.22$.^[ref]

In the usual scalar circular-pupil approximation for a fluorescence microscope, the corresponding object-plane Rayleigh criterion isHere ${\lambda }_0$ is the vacuum emission wavelength, and the object-side numerical aperture already includes the medium's refractive index. Do not divide ${\lambda }_0$ by $n$ again. For small cone angles in air, $\mathrm{NA}\simeq D/(2f)$ and image-plane radius $f{\theta }_{\mathrm{R}}$ agrees with this form. The assumptions include a single wavelength, ideal optical focus, and mutually incoherent emitters. High numerical aperture can require a vector description with polarization of an electromagnetic wave and emitter orientation; confocal microscopy has its own excitation-and-detection response. The same $0.61$ formula therefore does not describe every microscope or illumination method.^[ref]

In this original model, ${\lambda }_0=550\mathrm{nm}$ and numerical apertures $0.25$ and $0.50$ give first-zero radii $1.342\mu \mathrm{m}$ and $0.671\mu \mathrm{m}$. Each point spread function is divided by its own peak to compare shape. The pupil axes are transverse wave vector components multiplied by ${\lambda }_0/(2\pi )$, so pupil radius equals numerical aperture. A wider pupil accepts a wider range of transverse wave vectors, producing a narrower image. Increasing magnification alone enlarges the same spot and does not reduce its object-plane radius.

The Rayleigh criterion is a useful visual convention, not an absolute information barrier. For equal incoherent sources with total detected radiant flux $F$ and separation vector $\mathbf{s}$, the ideal image isBelow the Rayleigh criterion this still differs from $Fp(\mathbf{x})$; for small $\mathbf{s}$, symmetry cancels the linear change and the first change is quadratic in separation. Estimating two unknown source positions is then increasingly sensitive to noise, background, relative brightness and uncertainty in the point spread function. Localizing one isolated source and recovering an arbitrary extended object are different inference tasks. Measurements that separate optical modes can also extract separation information differently from direct image light intensity measurement.^[ref]

There remains a real limit for arbitrary images in this model: the optical transfer function is zero above the transmitted spatial frequency band. Its cutoff for scalar incoherent imaging is ${\nu }_c=2\mathrm{NA}/{\lambda }_0$ in object-plane cycles per unit length. The Nyquist–Shannon sampling theorem therefore suggests object-referred detector pixel pitch $p_{\mathrm{pix}}\le {\lambda }_0/(4\mathrm{NA})$, with finer sampling providing margin. Finite detector pixels also integrate over their area, suppressing contrast; coarse sampling can cause aliasing. Finer sampling cannot alone recover spatial frequencies removed by the optics, and Fourier deconvolution amplifies noise where the optical transfer function is small. Photon shot noise, read noise, optical aberrations and defocus can all worsen practical discrimination. A useful resolution claim therefore reports the object model, criterion, wavelength, numerical aperture, sampling and signal-to-noise ratio in photon counting.

The 2002 experiment trapped a $6.3\mu \mathrm{m}$ bead in water with a $980\mathrm{nm}$ laser, a $100\times$, numerical aperture $1.3$ objective and a piezo-driven microscope stage. A quadrant photodiode tracked bead position at $1\mathrm{kHz}$ with approximately $500\mathrm{Hz}$ bandwidth and reported $15\mathrm{nm}$ resolution. Stage translation drove water past the fixed optical trap at $1.25\mu m/s$. Weak trapping prolonged the transient; stationary recording before translation established the position reference. Stokes drag law calibration was checked against the bead's measured diffusion coefficient. ^[ref]

From calibrated displacement and calibrated harmonic optical trap force, the experimenters evaluated the dimensionless driven-bead dissipation functionalAmong 540 trajectories, negative values became less frequent with duration. Figure 2 compared the negative/positive count ratio with $⟨e^{-{\Sigma }_t^{\mathrm{drive}}}{⟩}_{{\Sigma }_t^{\mathrm{drive}}>0}$; its agreement tested the paper's integrated transient relation. The detailed log-ratio plot in Figure 4 used simulated particle sample trajectories, not bead measurements. Near-zero sign discrimination and stage-start transients limited the earliest points. ^[ref]

For any continuous probability distribution satisfying the same-law relation, integration over $s>0$ yieldsThis conditional relation explains the comparison without equating it to the unconditional integral fluctuation theorem. The experiment supports a specified driven-system relation; it does not establish a universal same-law symmetry for any definition of heat or entropy production. In particular, a transient work-like functional should not silently acquire the actual-distribution endpoint term in finite trajectory entropy production. Negative realizations are compatible with positive mean dissipation and the Second law of thermodynamics; they do not provide a cyclic machine with reliable work output from a single thermal reservoir.

Empty magnification enlarges an image without revealing additional specimen detail. Adequate angular magnification can make existing detail visible to the eye, but it cannot undo the objective's diffraction blur. For equally bright, mutually incoherent optical point sources and an ideal circular optical pupil, a commonly used lateral Rayleigh criterion in object space iswhere $\lambda$ is the vacuum wavelength, $n$ is the specimen-medium refractive index, and $\alpha$ is the admitted half-angle. For this model, $\lambda =550\mathrm{nm}$ and $\mathrm{NA}=0.25$ give $d_{\mathrm{R}}\simeq 1.342\mu \mathrm{m}$; changing only numerical aperture to $0.50$ gives $0.671\mu \mathrm{m}$. Changing only the eyepiece leaves this object-space scale unchanged. Illumination, optical coherence, optical aberrations and specimen visibility matter in real instruments; this criterion is not a universal threshold.^[ref]

The geometric reason is especially simple. If a fixed recorded image is enlarged by a factor $q>0$, a feature separation $s$ and a blur width $b$ become $qs$ and $qb$. Their ratio remainsThis is the lower-panel construction in the diagram. A change to the microscope objective can change numerical aperture and hence the specimen detail captured; a display zoom changes how large that captured detail looks. Microscope scale calibration, diffraction-limited resolution and angular magnification are three complementary properties to report.

Consider a planar circular aperture of diameter $D$ in constant refractive index $n$. Suppose every position in its area $A=\pi D^2/4$ is filled with directions inside the same rotationally symmetric cone about the unit normal, with half-angle ${\theta }_{\max }$. Using spherical coordinates, the exact etendue iswhere $\mathrm{NA}=n\sin {\theta }_{\max }$ is the appropriate side's numerical aperture. The ordinary cone solid angle is $2\pi (1-\cos {\theta }_{\max })$; its cosine-weighted value is $\pi {\sin }^2{\theta }_{\max }$. A full forward hemisphere therefore gives $G=n^{2}A\pi$, rather than $2\pi n^{2}A$. ^[ref]

For an original model example, choose $n=1$, $D=10\mathrm{mm}$, and ${\theta }_{\max }=30^{\circ }$. ThenIf the radiance is uniform across this filled bundle and equals $L=100\mathrm{W}{\mathrm{m}}^{-2}\mathrm{s}{\mathrm{r}}^{-1}$, its radiant flux is $\Phi =LG/n^2\approx 6.1685\mathrm{mW}$. Neither the geometry nor the chosen radiance is an experimental measurement.

A useful motivating experiment moves a bead emitting fluorescence through the selected optical focus and records the detector signal against position. Measurement of point-spread function (PSF) for confocal fluorescence microscopy by InCheon Song and others (2005, §2 and Fig. 1) describes a $543.8\mathrm{nm}$ laser delivered through a single-mode optical fiber, collimation, beam-size adjustment and a dichroic beam splitter. A $100\times$, numerical aperture $1.3$ oil-immersion objective optical lens excites slide-mounted fluorescent beads and collects their emission. Emission passes a filter and collecting optical lens, then a pinhole, before an avalanche photodiode. A three-dimensional positioning stage moves the specimen. The apparatus also contains a phase plate for deliberately modified responses.^[ref]

The published comparison uses nominal bead diameters $0.17,0.5,1\mu \mathrm{m}$: larger beads give broader measured spots. This is a control on the finite emitter, because the image is the convolution of bead emission with the confocal point spread function. The smallest bead has weaker signal relative to noise; incorrect bead size, defocus, bleaching and optical aberrations limit inference from a restored response. The reported bead-size comparison concerns lateral responses; it is not a measured pinhole-size series or a measured sheet-sectioning curve.^[ref]

To test axial pinhole selection with this kind of apparatus, the following is a proposed extension, not an additional result from that paper. Choose an isolated bead small relative to the response, centre it laterally, then step $z$ through optical focus while recording background-subtracted signal at several pinhole settings. Hold excitation power, dwell interval, detector gain, emission band and sampling fixed. Preserve raw peaks before also plotting peak-normalized traces. A lower peak and faster relative decay with axial displacement would support the paired-selection mechanism; fitting only normalized widths would miss the throughput cost.

Measure blocked-excitation background, include a bead-free region, avoid detector saturation, and repeat scans in reversed order to reveal drift or bleaching. Check pinhole centring separately, since misalignment can mimic signal loss. Record bead diameter, immersion medium, refractive index, numerical aperture, wavelengths and axial step. A finite bead is an approximation to an optical point source; a point trace does not certify confocal optical sectioning of a whole fluorescent sheet. Depth-dependent spherical aberration, scattering and sample motion can broaden or distort the response. Increasing magnification or making a display brighter does not correct those effects.

Projection lithography using a liquid between the final lens and wafer to increase numerical aperture through its refractive index, with stringent optical and chemical compatibility requirements.

The specimen-facing optical lens assembly of an optical microscope. A microscope objective collects light from the specimen and sets the object-side numerical aperture. In a finite-conjugate microscope it forms the intermediate microscope image directly; in an infinity-corrected microscope a tube lens completes that image formation. Its label alone does not calibrate a specimen length.

A multimode optical fiber supports several guided normal modes at the operating wavelength. Different mode populations can change its output cone, so launch conditions matter when measuring numerical aperture or interpreting focal-ratio degradation.

The numerical aperture is $n\sin \alpha$, where $n$ is the refractive index of the medium between object and objective, and $\alpha$ is the half-angle of the admitted cone of light. Specify object-side or image-side numerical aperture; the microscope convention usually means the object side.^[ref]

The optical fiber acceptance angle is the largest launch half-angle admitted by the specified guide model. For a flat entrance face perpendicular to the optical axis, external refractive index $n_0$, and a straight step-index optical fiber, Snell's law at the entrance givesAt the core–cladding interface, the critical angle for total internal reflection $i_c$ satisfiesFor a meridional ray, strict total internal reflection requires $i>i_c$. At the limiting geometry $i=i_c$, the refracted light ray is tangent to the interface, soThis identifies the numerical aperture with an entrance cone under these assumptions. With $n_0=1$ for air, the conventional inclusive boundary condition isfor a nonnegative launch angle $\theta$; a practical launch stays inside the boundary. If $\mathrm{NA}>n_0$, this ideal model imposes no further restriction within the forward hemisphere, and $\arcsin (\mathrm{NA}/n_0)$ must not be used. The light ray must also intersect the optical fiber core: a suitable angle alone does not correct a misplaced beam.

For an original example, take $n_1=1.50$, $n_2=1.48$ and air at the entrance. ThenThe internal limiting angle is only ${\varphi }_{\max }\simeq 9.37^{\circ }$, while the critical angle for total internal reflection is $i_c\simeq 80.63^{\circ }$. A $10^{\circ }$ launch has $\varphi \simeq 6.65^{\circ }$ and $i\simeq 83.35^{\circ }$, so it satisfies total internal reflection. A $20^{\circ }$ launch has $\varphi \simeq 13.18^{\circ }$ and $i\simeq 76.82^{\circ }$, so it fails this test.

This cone is a geometrical optics result for meridional rays. A single-mode optical fiber requires the spatial overlap of the incident electromagnetic wave with its guided normal mode; its numerical aperture does not impose an exact angular cutoff on arbitrary beams. Diffraction, finite cladding, and bends require a physical optics description.^[ref]

MIT's Optical Waveguides and Fibers, Laboratory Exercise No. 7 (Fall 2005), describes a teaching apparatus. A cleaned, flat-ended multimode optical fiber receives helium–neon laser light through a microscope objective optical lens; a micropositioner aligns the input. The launch deliberately overfills the fibre's cone. A beam splitter sends a sample to a reference photodetector to track laser fluctuations. The fixed output faces a screen or a photodetector on a rotation stage with angular measurement calibration. Readings every two degrees, divided by simultaneous reference readings, give an angular profile. Repeating with an underfilled launch is a control: too few excited normal modes underestimate numerical aperture. Optional index-matching fluid removes cladding-carried light. Bending the fibre reveals leakage and reduced output on an oscilloscope. The handout supplies no measured trace or absolute detector measurement calibration, and this angular scan does not determine loss per kilometre.^[ref]

An optical microscope uses light and optical lens assemblies to reveal small structures, such as the boundaries of biological cells or defects in a manufactured surface. In a compound optical microscope, the microscope objective supplies an enlarged real image; the microscope eyepiece makes that intermediate microscope image subtend a larger viewing angle. The transverse magnification of the first stage and the angular magnification of the second answer different questions.^[ref]

Consider an original ideal finite-conjugate microscope in air, with both optical lens assemblies treated as converging thin lens elements in the paraxial approximation. Choose objective focal length $f_o=10\mathrm{mm}$ and object distance $u_o=12\mathrm{mm}$. The thin lens equation and signed transverse magnification givePlace a microscope eyepiece of focal length $f_e=25\mathrm{mm}$ a further $25\mathrm{mm}$ beyond the intermediate microscope image. The objective-to-eyepiece separation is therefore $85\mathrm{mm}$. Each object point then supplies a parallel outgoing bundle of light rays, appropriate to a relaxed eye focused at infinity. Define the unaided reference as the same object viewed at distance $D=250\mathrm{mm}$; this is a rating convention, not a universal property of eyesight. The eyepiece angular magnification and total signed angular magnification areFor instance, a hypothetical object height $h_o=0.020\mathrm{mm}$ becomes $h_i=-0.100\mathrm{mm}$ at the intermediate microscope image. The signed viewing angles are approximately $h_i/f_e=-0.004\mathrm{rad}$ with the instrument and $h_o/D=0.00008\mathrm{rad}$ unaided. Their ratio is $-50$: enlarged and inverted. These are stipulated model dimensions, not measurements or specifications of a modern commercial optical microscope.

The attraction of an optical microscope is that seeing can become measuring: a stage micrometer supplies a known object-space length, and microscope scale calibration converts an image readout into a specimen length. This also separates a large-looking image from a trustworthy size estimate. Meanwhile, diffraction-limited resolution depends on numerical aperture and wavelength; empty magnification enlarges an existing blur without revealing additional structure.

The weak phalloidin signal could include sparse or very short residual actin filaments, together with nonspecific background; it is not a direct image of G-actin. phalloidin preferentially binds filamentous actin, so genuinely specific residual signal would indicate some polymeric actin. Long exposure also amplifies autofluorescence, out-of-focus light and nonspecific staining. Vehicle controls, unstained biological cells, matched acquisition settings and background subtraction are needed before assigning a small residual signal to actin filaments.

For wavelength $\lambda$ and numerical aperture $\mathrm{NA}$, the Rayleigh criterion gives a conventional lateral resolution of roughly $d\approx 0.61\lambda /\mathrm{NA}$, typically a few hundred nanometres under these conditions, far larger than the roughly 7-nm actin filament diameter. It cannot resolve every short actin filament, distinguish all closely spaced actin filaments, or infer polymer mass directly from the displayed intensity. The tenfold exposure change is not itself a calibrated tenfold decrease in F-actin. Fixation, dye accessibility and detector saturation introduce further uncertainty.

Super-resolution fluorescence microscopy could localize remaining labelled structures more precisely, while electron microscopy or cryo-electron tomography could test ultrastructure, subject to preservation and sampling limits. Biochemical separation of polymeric and soluble actin, with the mass-balance and aggregation controls already discussed, supplies an independent population measurement. A live actin reporter can test dynamics but must be checked for perturbation of assembly. Agreement between these approaches would be stronger than concluding complete actin filament absence from weak conventional staining alone.

The raised-index region has $n_1=1.01(1.50)=1.515$, whereas $n_2=1.50$. Therefore its numerical aperture factor is $\sqrt{n_1^2-n_2^2}=0.212662$. With the printed rounded cutoff,ThusSlower scanning increases the written radius and makes the guide multimode; faster scanning makes it single-spatial-mode in this idealization, provided a physically meaningful positive core radius remains. Using $j_{0,1}=2.404825\dots$ gives $s_c\simeq 586.2\mathrm{mm}{\mathrm{s}}^{-1}$. This numerical threshold extrapolates the supplied empirical radius law; its experimental range is not given.

Finite numerical aperture and diffraction remove high spatial frequencies from a mask image. Neighboring features modify the aerial intensity, and the photoresist threshold turns this blurred intensity into biased edges, shortened lines and rounded corners. optical proximity correction deliberately predistorts the mask: line-end extensions and small serifs can compensate for local loss of exposure, while other edges are moved back where exposure spreads too far. The correction is calibrated for illumination, resist and focus; it does not restore arbitrarily high lost spatial frequencies.

double patterning separates a dense target pattern into two sparser patterns, each printable within the single-exposure process window. Two exposures with an intervening pattern-fixing or transfer step combine their features on the wafer. For regular lines, alternating line assignment can halve the final pitch while each exposure retains a larger pitch. optical proximity correction corrects local image bias; double patterning divides the density burden between exposures. Overlay error and the minimum printable isolated feature still constrain the result. Simply exposing the same unmodified resist twice without an appropriate process separation does not give the same independent thresholding.

A useful scale estimate is $d\sim k_1\lambda /\mathrm{NA}$, where $\mathrm{NA}=n\sin \alpha$. Five complementary developments are immersion optics, illumination shaping, phase-shifting masks, computational proximity correction and multiple patterning. They address different parts of the process rather than repeal diffraction.

Immersion lithography raises numerical aperture by putting a compatible high-index liquid between the final lens and wafer. Off-axis, annular or dipole illumination places useful diffraction orders within the projection pupil and improves contrast for selected pattern directions.

A phase-shift mask uses controlled optical phase differences to sharpen destructive interference around selected features. Optical proximity correction modifies mask edges and adds assist features to compensate pattern-dependent image formation, resist and etch biases.

Multiple patterning divides a dense final layout into more widely spaced optical patterns, or uses spacer-based pitch division after an optical definition step. Overlay, etch control and pattern decomposition are then critical. For example, with $\lambda =193\mathrm{nm}$, $\mathrm{NA}=1.35$ and $k_1=0.25$, a single-exposure scale is roughly $36\mathrm{nm}$; obtaining dense features of order $20\mathrm{nm}$ commonly requires pitch multiplication rather than attributing everything to one exceptionally sharp aerial image.

These resolution enhancements also require suitable resist contrast, focus control and metrology. The manufacturer's description explains the numerical-aperture benefit of immersion; no claim about a particular 2018 production process is inferred from its current product pages.^[ref]

For a positive photoresist, let $D_0$ be the largest exposure preserving the initial film thickness and $D_c$ the clearing dose. The photoresist contrast is $\gamma =1/{\log }_{10}(D_c/D_0)$. An aerial image $I=\bar{I}[1+M\cos (2\pi \nu x)]$ can clear its maxima while retaining its minima only ifThus $M_c=0.6991$ and $0.2006$ for the lower- and higher-contrast materials. Reading those levels on the supplied modulation transfer function gives maximum normalized spatial frequencies approximately $\nu /{\nu }_0=0.40$ and $0.60$, respectively. The stated partial coherence is already represented by that curve; do not multiply the read frequencies by another coherence factor.

The unambiguous pattern dimensions are full pitch $P=1/(x{\nu }_0)$ and equal line/space width $w=P/2$, where $x$ is the read normalized frequency. To convert the paper's Rayleigh-frequency label into lengths, explicitly use the circular-pupil Rayleigh criterion $d_R=0.61\lambda /NA$ and ${\nu }_0=1/d_R$. With the ArF vacuum wavelength $193\mathrm{nm}$ and the stated numerical aperture, $d_R=235.5\mathrm{nm}$, givingThe higher-contrast material resolves a line/space feature about two-thirds as wide. The pitch and half-pitch distinction matters: the point-separation Rayleigh criterion is not itself an equal-line/space width. If a course defines ${\nu }_0$ as an optical cutoff rather than $1/d_R$, use that specified frequency in $w=1/(2x{\nu }_0)$; the normalized graph readings and their ratio are unchanged. No absolute clearing dose is provided.

An immersion liquid increases numerical aperture through $NA=n\sin \alpha$, if the optics can use the same ray angle $\alpha$ inside a transparent, chemically compatible liquid of refractive index $n$. With unchanged $k_1$ and vacuum wavelength, the width scales as $R_{\mathrm{imm}}=R_{\mathrm{air}}/n$ at fixed angle. If the two empirical formulae printed in the question are used literally with fixed $k_2$, they give $DO{F}_{\mathrm{imm}}=300/n^2\mathrm{nm}$.

For a physical axial displacement inside the medium, however, the familiar paraxial defocus range contains an additional index factor: $DOF\propto n{\lambda }_0/N{A}^2$. That convention gives $DO{F}_{\mathrm{imm}}/DO{F}_{\mathrm{air}}\simeq 1/n$ at fixed ray angle, or an approximately $n$-fold larger depth of focus at fixed $NA$ and resolution. Thus immersion can improve lateral resolution, while its focus benefit depends on what is held fixed and on the axial-focus convention. It cannot improve resolution merely by inserting $n$ into $NA$ while ignoring optical redesign, absorption or resist compatibility. No liquid index is supplied for a numerical prediction.