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A fluorescence microscope maps photon emission to an image. Excitation light passes through a fluorescence excitation filter and reflects from a wavelength-selective beam splitter into the objective optical lens. Returning fluorescence passes through that beam splitter and a fluorescence emission filter to the photodetector. Rejecting excitation makes faint emission visible.^[ref]

An ordinary image records emission-band counts at each pixel. Adding pulse-delay measurements gives a fluorescence lifetime map; scanning emission wavelength gives spectral information instead. Confocal microscopy adds spatial rejection and confocal optical sectioning, as developed in its own branch. An unlabeled specimen under the same illumination reveals intrinsic fluorescence and excitation leakage; excitation-blocked frames reveal detector background. A blank cannot perfectly predict background in a differently prepared specimen.

Fluorescence quantum yield counts emitted fluorescent photons per absorbed excitation photon. It differs from quantum efficiency, which describes the photodetector.^[ref]

For an original constant-rate model, let $k_{\mathrm{r}}$ be the radiative decay rate and $k_{\mathrm{nr}}$ the sum of competing rates without fluorescent photon emission. An initial population $N_0$ in the emitting excited state obeys the rate equationIntegrating $k_{\mathrm{r}}N(t)$ over all time givesHere every absorbed excitation is assumed to reach this emitting population; additional losses before that stage require another factor. Under steady weak excitation, absorbed photon rate $R_{\mathrm{abs}}$ and total detection fraction $\eta$ give $C=\eta \Phi R_{\mathrm{abs}}$. The count rate therefore mixes excitation, yield, and detection, whereas $\tau$ specifies a decay timescale.

The focal ratio, or f-number, of a converging optical instrument focused at infinity is the dimensionless quantitywhere $f>0$ is effective focal length and $D$ is the on-axis entrance pupil diameter. The notation f/4 means $N=4$, not $f=4$ in a particular unit. A $100\mathrm{mm}$ focal length with $D=25\mathrm{mm}$ gives f/4. Closing the aperture stop until $D=12.5\mathrm{mm}$ gives f/8 while retaining the same focal length. The mechanical opening need not equal $D$. ^[ref].

For a thin lens in air with a circular aperture stop at the thin lens, the converging bundle from a distant on-axis point has half-angle $\alpha$ satisfyingThe last step uses the small-angle approximation. For a compound optical lens, the cone is set by the image-side optical pupil and its distance from the receiving plane; the simple triangle is not an exact high-angle rule. At finite object distance a thin lens instead gives $v=f(1+|m|)$, by its imaging equation and transverse magnification, so its working cone corresponds to $v/D=N(1+|m|)$. Nominal focal ratio therefore does not alone specify close-range image irradiance. ^[ref].

Two useful scalings follow under stated conditions. For a fixed uniform incident beam and unchanged transmission, collected radiant flux scales as $D^2$. For an extended source with fixed radiance, the central image irradiance scales approximately as $N^{-2}$ in the paraxial approximation: the solid angle of the cone scales as $(D/f{)}^2$. For an ideal uniformly illuminated unobscured circular entrance pupil, the first dark ring of the point spread function has angular radius and focal plane radiusHere $\lambda$ is wavelength. These are first-zero radii, not diameters or a universal rule for resolving arbitrary objects. A proof outline evaluates the circular aperture's Fraunhofer diffraction using its Fourier transform, then uses the first zero of the resulting Bessel function. ^[ref].

At $\lambda =0.55\mu \mathrm{m}$, the example's f/4 and f/8 first-ring radii are $2.684$ and $5.368\mu \mathrm{m}$. Closing the aperture stop halves $D$, quarters collected radiant flux and doubles this diffraction radius. It can also reduce optical aberrations by excluding outer light rays; actual sharpness reflects both effects. This explains why a smaller aperture need not produce a sharper image. ^[ref].

A proposed optical bench check puts a fixed collimator before a converging thin lens and a photodetector large enough to collect the entire image behind it. Compare two centred aperture stops of known diameters while fixing source output, detector gain and integration time. The integrated signal tests the $D^2$ collection law; a spatially resolved optical camera at the focal plane can separately measure the point spread function. Repeat a dark reading and the first aperture stop, and check that neither the incoming beam nor detector clips either bundle. A resolved pinhole source, imperfect collimation, optical aberrations and detector pixel size can dominate the apparent spot width; a single peak reading does not measure total collected radiant flux.

Retaining events with a detection in each of four channels within a specified timing window. For exactly four incident photons and ideal photodetectors, a click in every channel requires one photon per channel. More photons, losses and detector timing introduce additional qualifications.

GW150914 is the first directly detected gravitational wave, interpreted as a binary black hole inspiral, merger and ringdown. Its discovery paper reports a peak projected gravitational wave strain of approximately $10^{-21}$ and a combined matched filter signal-to-noise ratio of 24. The inferred source-frame component masses were $36_{-4}^{+5}$ and $29_{-4}^{+4}$ solar masses, with 90 percent credible intervals. These are model-dependent estimates from a detector network.^[ref]

The interesting measurement is a chain: curvature changes relative light travel times, wave interference turns those changes into a photodetector signal, calibrated records from separate sites constrain a common source, and statistical inference tests competing explanations. The following original models explain that chain; the plotted time series are stipulated calculations, never measured GW150914 data.

Robert Hanbury Brown and Richard Q. Twiss sought angular sizes through correlations of detected light intensities. Their method multiplies detector outputs after detection, whereas a Michelson interferometer combines fields before detection.^[ref]

A monochromatic discharge source illuminated a half-silvered beam splitter and two photodetectors. The averaged current product showed an excess near coincidence. Moving one detector introduced a delay that removed it, supplying a control. Slow response weakened the peak. This photon bunching is compatible with fluctuating classical light intensity.^[ref]

Holography records the wave interference between a holographic object wave and a known holographic reference wave, then uses that record to reconstruct a wavefront. The record is a hologram. A photographic plate or photodetector measures light intensity; the relative wave phase is encoded in the positions and contrast of interference fringes, rather than measured directly as an optical oscillation. Reillumination makes the recorded pattern act as a diffraction grating that produces an image-bearing field.

The useful change of viewpoint is to preserve a field that can propagate again, instead of only a focused brightness picture. Recovered complex amplitude permits refocusing with the Fresnel propagator; a spatially extended hologram can also supply different viewing directions. The recoverable information is limited by the collected aperture, recorded spatial frequencies, optical coherence and separation of unwanted diffraction orders.

Infrared is electromagnetic radiation with wavelengths longer than the red end of ordinarily visible light. A useful named convention is the CIE range $780\mathrm{nm}$ to $1\mathrm{mm}$; the approximate visible range $400$–$700\mathrm{nm}$ used in some teaching texts puts the boundary nearer $700\mathrm{nm}$. ^[ref]. Neither number is a sharp physical cutoff of human vision. The CIE divisions IR-A, IR-B and IR-C are respectively $0.78$–$1.4\mu \mathrm{m}$, $1.4$–$3\mu \mathrm{m}$ and $3$–$1000\mu \mathrm{m}$; the boundaries of near-, middle- and far-infrared vary between applications. ^[ref].

Use vacuum wavelength $\lambda$ when comparing these ranges. With frequency $\nu$, speed of light $c$, Planck constant $h$ and photon energy $E$,Thus the CIE infrared interval corresponds approximately to $3.00\times 10^{11}$–$3.84\times 10^{14}\mathrm{Hz}$ and $1.99\times 10^{-22}$–$2.55\times 10^{-19}\mathrm{J}$ per photon, with the smaller energy at the longer wavelength. A $940\mathrm{nm}$ infrared photon has $E\simeq 2.11\times 10^{-19}\mathrm{J}$, whereas a $254\mathrm{nm}$ ultraviolet photon has $E\simeq 7.82\times 10^{-19}\mathrm{J}$. These are calculations from $hc/\lambda$, not measurements of a particular source. A television remote is a familiar approximately $940\mathrm{nm}$ source; its information is carried by variations in the emitted radiant flux. ^[ref].

Detection and visual perception answer different questions. A photodetector can respond to infrared even when an observer perceives no light; a thermometer detects an increase in temperature after photon absorption. Conversely, a small readout can reflect a small spectral responsivity, rather than absence of incident electromagnetic radiation. Images made from infrared readouts use display colors chosen by the imaging system; those colors are not the colors of the incoming infrared.

The important microscopic mechanisms also differ. In infrared spectroscopy, photon absorption often increases the energy of a normal mode in which chemical bonds stretch or bend. The corresponding optical spectrum helps distinguish molecules by their motions. For the usual electric dipole mechanism, the motion must change the electric dipole moment; matching the frequency alone is not sufficient. ^[ref]. Electronic processes can also emit or absorb infrared, so it is not exclusively radiation from molecular motion. ^[ref]; ^[ref].

Infrared is especially useful for observing thermal radiation from objects near everyday temperatures. For an ideal $300\mathrm{K}$ blackbody, Wien's displacement law gives a wavelength peak near $9.66\mu \mathrm{m}$. A thermal camera's readout nevertheless depends on emissivity, reflected surroundings and transmission along the path, as well as temperature. ^[ref]. A source emitting infrared need not be hot: an infrared remote is useful precisely because a photodetector can distinguish its signal from a background. Visible light and ultraviolet can also cause heating when absorbed; “infrared” names a wavelength range, not a unique form of heat.

Herschel's 1800 discovery made the distinction between sight and detection experimentally concrete. His report describes a optical prism spreading sunlight across a white, ruled receiving surface, with three thermometers at different positions near the end of the visible optical spectrum. He observed heating beyond the red edge where no visible color appeared. The result established that sunlight contained invisible radiation less strongly refracted by that optical prism than the visible red; it did not measure a photon energy or establish today's infrared boundaries. ^[ref]; ^[ref].

A documented modern version by Caltech/IPAC uses a glass optical prism, white paper in a shaded box and three blackened liquid-filled thermometers. Initial readings in shade provide each thermometer's baseline; subsequent readings compare blue, yellow and just beyond red after the bulbs have had time to warm. The measured quantity is the rise in temperature, not a “temperature of a color”. The lesson reports the expected largest rise beyond visible red and specifically discusses matching the bulb coatings and thermometer calibration. Clouds and changes in the projected optical spectrum affect the comparison. ^[ref].

For a stronger modern interpretation, an additional shaded thermometer would monitor ambient drift, exchanging bulbs between positions would test differences between thermometers, and blocking the incident beam would distinguish beam-dependent heating from warmed supports or air. These are suggested controls, not claims about Herschel's historical procedure. Thermal conduction through the mounting and convection can change the readout. A broad bulb also samples a range of wavelengths; the optical prism's transmission and optical dispersion, the bulb's absorption and heat loss all affect the position of the largest rise. In particular, the result does not show that an infrared photon carries more energy than a visible photon.

A simple analytical illustration makes those limitations explicit. Let $\theta =T-T_{\mathrm{amb}}$ be the bulb's excess temperature, $C$ its heat capacity, $G$ its effective heat-loss coefficient in $\mathrm{W}{\mathrm{K}}^{-1}$, and $P_{\mathrm{abs}}$ the absorbed power. Assume one uniform bulb temperature, constant ambient temperature, constant $C,G$, and losses linear in $\theta$. Thenfor a beam switched on at $t=0$ with $\theta (0)=0$. After switching the beam off at $t=t_s$, $\theta (t)=\theta (t_s)e^{-(t-t_s)/\tau }$. The exponential function follows by solving this linear differential equation. The steady excess temperature measures $P_{\mathrm{abs}}/G$; it is not a direct measurement of the optical spectrum. With illustrative $C=0.020\mathrm{J}{\mathrm{K}}^{-1}$ and $G=0.0010\mathrm{W}{\mathrm{K}}^{-1}$, $\tau =20\mathrm{s}$. Absorbed powers of $1.0$ and $0.5\mathrm{mW}$ give steady rises of $1.0$ and $0.5\mathrm{K}$, regardless of which wavelengths supplied that absorbed power.

The upper schematic represents the logic of the optical prism experiment; its light ray positions are not a calibrated wavelength scale. The lower curves use the illustrative heat-balance model, with the beam blocked at $60\mathrm{s}$; they are not historical or modern measured data. The beauty of the experiment is that a simple change of detector extends observation beyond what the eye can perceive.

With fixed spectral resolving power, wavelength interval, diffraction grating geometry, photodetector sampling and collimator focal ratio, a beam diameter scales as the spaxel width $\theta$, but the optical camera focal length stays fixed. A simple on-axis beam-envelope model therefore gives collimator and disperser volumes proportional to ${\theta }^3$ and optical camera volume proportional to ${\theta }^2$. With a fixed number of spaxels per photodetector, replication multiplies these volumes by $M\propto {\theta }^{-2}$. A fixed photodetector field adds transverse clearances and changes the small-$\theta$ limit; these exponents are not universal mechanical-volume laws.

Alfred Kastler's 1966 lecture recalls his 1950 proposal and distinguishes the early ${}^{23}\mathrm{Na}$ atomic beam work from later sealed-vapour experiments. Its Fig. 5 shows subsequent sodium radio wave resonances, including multiple-photon transitions.^[ref]

The 1952 demonstration by Jean Brossel, Alfred Kastler and Jacques Winter used a sodium atomic beam, lamp light with circular polarization and fluorescence readout. Jean Brossel was a central experimental contributor; Alfred Kastler received the 1966 Nobel Prize in Physics alone.^[ref][ref]

The lecture also describes a ${}^{199}\mathrm{Hg}$ experiment: a pumping beam with circular polarization prepares the vapour, while a perpendicular probe converts transverse magnetic dipole moment Larmor precession into modulated absorption at a photodetector. Interrupting pump and RF together exposes decaying free Larmor precession. Its envelope measures transverse relaxation under that model; it is distinct from the sodium atomic beam fluorescence signal.^[ref]

MIT's later rubidium experiment pumps natural ${}^{85}\mathrm{Rb}$/${}^{87}\mathrm{Rb}$ with a discharge lamp filtered to D1 ($5S_{1/2}\to 5P_{1/2}$, near $795\mathrm{nm}$). A polarizer and quarter-wave plate precede a heated rubidium/neon cell. Helmholtz coils set a weak static magnetic field. Separate transverse RF mixing increases absorption, measured by photodiode transmission.^[ref]

Compare resonant and detuned RF with the optical beam fixed, then change the calibrated static magnetic field: a true magnetic sublevel resonance should move with its Zeeman splitting. Lamp drift, stray magnetic fields, imperfect circular polarization, optical attenuation and RF power broadening can change the observed signal. Consequently, a transmission dip alone does not determine every sublevel population. These are measurement-design considerations, not additional reported historical observations.

An ideal single-round-trip Michelson interferometer converts differential length into phase difference $\delta \varphi =4\pi \Delta L/\lambda$. If its dark-port power scale is $P_0$ and its static phase offset is ${\varphi }_0$, thenExactly at a dark fringe, ${\varphi }_0=0$, power is quadratic in small displacement and loses its sign. A deliberate offset supplies an optical reference field: interference with the small signal field produces a linear term. Equivalently, $|E_{\mathrm{ref}}+E_{\mathrm{sig}}{|}^2$ contains $2\mathrm{Re}(E_{\mathrm{ref}}^{\ast }{E}_{\mathrm{sig}})$. The photodetector measures power, not a signed displacement directly.

Advanced LIGO uses this DC homodyne principle with a small differential arm offset and an output mode cleaner. Its feedback control maintains optical resonance and alignment, using actuation distributed over the suspension stages.^[ref] The simple formula above omits resonant storage: arm and recycling cavities give a frequency-dependent optical gain and delay, so no constant pass-count multiplier describes the entire observing band.

To see why a locked detector can still measure a disturbance, use a linear feedback control model. Let $x_{\mathrm{ext}}$ be the external differential displacement, $d_{\mathrm{err}}$ the residual readout, $d_{\mathrm{ctrl}}$ the recorded control command, $C(f)$ the sensing response and $A(f)$ the actuation response. With the convention $x_{\mathrm{res}}=x_{\mathrm{ext}}-A{d}_{\mathrm{ctrl}}$, reconstruction givesHere tildes denote Fourier transforms; $C$ has readout units per metre and $A$ metres per command unit. Feedback suppresses residual motion but records the compensating command. Omitting that command would suppress the reconstructed low-frequency signal too. In the actual GW150914 experiment, a modulated auxiliary laser exerted calibrated radiation pressure on a mirror to measure the response; periodic excitations monitored calibration.^[ref]

Linear spectral dispersion is the derivative of the photodetector coordinate in the dispersion direction with respect to wavelength. Near the optical axis, $q=f_{\mathrm{cam}}d\beta /d\lambda$.

A monochromator selects a narrow, adjustable wavelength interval from an optical spectrum. Its finite spectral bandwidth matters when calibrating a photodetector's spectral responsivity in calibrated radiant-flux measurement.

An optical camera images incident beams on a photodetector; a spectrograph optical camera maps a diffracted direction into a position in its focal plane.

An optical photodetector detector pixel is a spatially sampled photosensitive element. Counts from distinct detector pixels have independent photon shot noise in the ideal model, but charge transfer, electrical coupling and resampling can produce covariance.

MIT's Optical Waveguides and Fibers, Laboratory Exercise No. 7 (Fall 2005), describes a teaching apparatus. A cleaned, flat-ended multimode optical fiber receives helium–neon laser light through a microscope objective optical lens; a micropositioner aligns the input. The launch deliberately overfills the fibre's cone. A beam splitter sends a sample to a reference photodetector to track laser fluctuations. The fixed output faces a screen or a photodetector on a rotation stage with angular measurement calibration. Readings every two degrees, divided by simultaneous reference readings, give an angular profile. Repeating with an underfilled launch is a control: too few excited normal modes underestimate numerical aperture. Optional index-matching fluid removes cladding-carried light. Bending the fibre reveals leakage and reduced output on an oscilloscope. The handout supplies no measured trace or absolute detector measurement calibration, and this angular scan does not determine loss per kilometre.^[ref]

An optical fiber cutback measurement estimates distributed optical fiber attenuation by comparing a long guide with the short input portion left after cutting the output end back. Keep the launch untouched. For the following original functional model, fix wavelength, source output, input alignment and collected output cone; subtract a dark reading and keep the photodetector in its linear range. Renewed end-face quality and changed normal mode populations can still spoil the comparison. The diagram describes a measurement principle, not Kao's historical short-glass apparatus.

Let $L_{\ell }>L_s$ be lengths in kilometres, $P_{\ell },P_s$ the corresponding positive dark-subtracted readings, and $C$ the same launch and detection factor. A uniform guide obeysHere ${\alpha }_{\mathrm{dB}}$ and its estimate are numerical values in $dB/km$. Dividing eliminates $C$; taking a logarithm proves the formula. A changed $C$ instead adds an error $10{\log }_{10}(C_s/C_{\ell })/(L_{\ell }-L_s)$. Reconnecting the launch between readings defeats this cancellation.

For original chosen values $L_s=0.1\mathrm{km}$, $L_{\ell }=2.1\mathrm{km}$, $P_s=100\mu \mathrm{W}$ and ${\alpha }_{\mathrm{dB}}=0.5dB/km$, the predicted long reading is $P_{\ell }=10010^{-0.1}\simeq 79.4328\mu \mathrm{W}$. The ratio gives $1\mathrm{dB}$ for the removed $2\mathrm{km}$ and hence $0.5dB/km$.

Suppose an unremoved additive detector background contributes $b=5\mu \mathrm{W}$ to both readings. Then the apparent coefficient isThis is a bias of an estimator: a common additive background pushes the ratio toward one, whereas a common multiplicative factor cancels. For independent small zero-mean errors with standard deviations ${\sigma }_s,{\sigma }_{\ell }$ on the dark-subtracted readings, a first-order Taylor expansion givesChoosing both standard deviations as $1\mu \mathrm{W}$ gives ${\sigma }_{\hat{\alpha }}\simeq 0.03491dB/km$. A shared estimated dark offset introduces covariance, so it requires a different uncertainty calculation. Near zero signal, the logarithm and this linear approximation become unreliable.

The beta barium borate crystal supplies spontaneous parametric down-conversion: a weak ultraviolet pump can produce a pair of lower-frequency photons, with their frequencies and wavevectors selected by conservation of energy and phase matching. A suitably aligned and compensated source makes the two polarization alternatives coherent, giving the stated Bell state. The crystal alone does not guarantee that every collected pair has that state.

The two polarizing beam splitters route horizontal polarization and vertical polarization to different ports. Take $H$ from arm $a$ to the trigger $T$ and $V$ to arm $c$. The ordinary beam splitter in arm $b$ is a lossless, polarization-independent 50:50 splitter, with the unused input in the vacuum state. It splits probability amplitudes, rather than measuring which output a photon takes. The half-wave plate changes the relative phase of its principal polarizations by $\pi$; orient it so that a $V$ photon in $c$ becomes $(H+V)/\sqrt{2}$ in $c^{\prime }$, up to a common phase. Finally, photodetectors absorb arriving photons and supply electrical clicks. They need not resolve photon number for the ideal four-photon, fourfold-coincidence calculation: a click at every detector already requires one photon at each.

Choose the relative wave phases so that the final polarizing beam splitter sends $H_{c^{\prime }}$ to $H_2$, $V_{c^{\prime }}$ to $V_1$, $H_d$ to $H_1$ and $V_d$ to $V_2$. Such phase conventions must be used consistently: an uncompensated relative phase would give a phase-shifted GHZ state.

The optical success probability of a fourfold GHZ source depends on the photon-number statistics of the assumed two-pair input. Let $q$ be the probability that arm $a$ contains one $H$ and one $V$, given two pairs. The beam splitter then puts one of the two $b$ photons into each output with probability $1/2$, and the final polarization-parity selection succeeds with probability $1/2$. With ideal photodetectors,For the normalized, fully overlapping two-pair component of polarization-entangled down-conversion used above, $q=1/3$, so the squared norm of the accepted component gives $P=1/12$. Treating the two emissions instead as independently labelled Bell pairs gives the commonly used bookkeeping estimate $q=1/2$ and $P=1/8$. This is a different pair-statistics approximation; it must not be obtained by dropping labels from an input state without renormalizing it. Genuine GHZ state coherence additionally requires erasing information about the pair of origin.

Let $P_2$ be the two-pair probability per pulse and $f$ the pulse repetition rate. The coincidence rate is $R=f{P}_{2}q/4$. With the usual order-of-magnitude estimate $P_2\simeq p^2$, the supplied $f=10^6{\mathrm{s}}^{-1}$ and $p=10^{-3}$ give $f{P}_2\simeq 1{\mathrm{s}}^{-1}$. The fully overlapping model gives $R\simeq 0.083{\mathrm{s}}^{-1}$, about one event per twelve seconds; independent-pair bookkeeping gives $R\simeq 0.125{\mathrm{s}}^{-1}$, about one per eight seconds. Thus the estimate is of order $10^{-1}{\mathrm{s}}^{-1}$ under these idealizations.

An exact prefactor cannot follow from the single-pair probability alone. For example, the pair-number statistics of polarization-entangled down-conversion in a single collected mode pair give $P_2\simeq 3p^2/4$ at weak gain, and hence $R\simeq f{p}^2/16=0.0625{\mathrm{s}}^{-1}$ with $q=1/3$. Multiple temporal or spectral modes change those statistics. Real collection losses and detector efficiencies further reduce the rate; they are excluded by the ideal calculation.

For heralded Bell-pair projection with two polarization parity filters, a Hadamard gate followed by computational-basis detection is a measurement in the diagonal polarization basis $|\pm ⟩=(|H⟩\pm |V⟩)/\sqrt{2}$. It can therefore be realized directly by a polarizing beam splitter at $45^{\circ }$ with a photodetector in each output: its transmitted and reflected clicks label $+$ and $-$. No separate Hadamard gate plate is required for this measurement.

Conditioning on one photon in each output of the first polarizing beam splitter implements the even $Z$-parity filterThe $45^{\circ }$ polarizing beam splitter similarly implements the even $X$-parity filter ${\Pi }_X^{46}=(I+X_4{X}_6)/2$. These are ideal coherent filters, with the optical phases chosen consistently. The all-zero photodetector record projects the upper outputs onto $⟨++{|}_{25}$ and the lower outputs onto $⟨00{|}_{46}$. The two effective bras areTherefore the unnormalized retained state isFor example, the simultaneous Bell contractions require the three pair bits to coincide, leaving only the $000$ and $111$ terms of the original pair expansion. Each surviving $|00{⟩}_{13}$ or $|11{⟩}_{13}$ amplitude is $1/(8\sqrt{2})$. ConsequentlyThis probability is conditioned on production of all three input pairs, but not yet on successful polarizing beam splitter routing. The routing probability itself is $⟨{\Pi }_Z^{25}{\Pi }_X^{46}⟩=1/4$: each nonidentity expectation vanishes on the relevant initial reduced state. Thus, if the question instead regards the routing postselection as already performed,These two denominators describe different conditioning. Neither includes the probability that the three crystals actually emit the required pairs, photodetector inefficiency or undesired multipair events.